Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Kinetics parameters of NDT and mutants
| Kinetics value | NDT | fNDT | fNDT-i1 | fNDT-i4 |
|---|---|---|---|---|
| Vmax (µM • hr−1) | 14.75 ± 0.03 | 17.28 ± 0.17 | 31.28 ± 0.4 | 29.57 ± 0.39 |
| Km (µM) | 1.865 ± 0.02 | 1.783 ± 0.06 | 1.289 ± 0.07 | 1.056 ± 0.21 |
| Kcat (hr−1) | 2.950 ± 0.006 | 3.456 ± 0.03 | 6.256 ± 0.08 | 5.914 ± 0.08 |
| Kcat/Km (hr−1 • µM−1) | 1.582 ± 0.02 | 1.940 ± 0.05 | 4.861 ± 0.18 | 5.842 ± 1.2 |
