Fig. 4.

Overexpression of miniagrin in heart after systemic delivery and improvement of cardiac histology. The AAV1 vector was injected i.p. in dy^w/dy^w neonates, and the mice [untreated homozygous mice dy/dy (Left), AAV-treated homozygous mice (dy/dy + AAV) (Center), and littermate WT control mice (Right)] were killed at 2 ^½ months of age. Cryo-thin sections of heart muscle were subjected to IF staining of agrin (a–c), agrin (red) counterstained with cell nuclei (green) (d–f), and collagen III (g–i). (Insets) Enlargement of a representative area of each panel to show more details. (Scale bars: 100 μm.)