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. 2005 Aug 10;102(34):12071–12076. doi: 10.1073/pnas.0502383102

Fig. 1.

Fig. 1.

Expression of BMAL1 during adipose differentiation. (A) White adipose tissue was excised from three male C57BL/6J mice (6 weeks old), mixed, and fractionated into adipocyte and a stromal–vascular (S.-V.) fractions. The total RNA was isolated, and the expression of BMAL1 mRNA was determined by Northern blot analysis. (B) Obese C57BL/6J mice were generated by feeding with a high-fat diet for 4 weeks. White adipose tissue was excised from three each of control mice and obese mice, and the expression of BMAL1 mRNA was determined by Northern blot analysis. (C) Differentiation of 3T3-L1 cells was induced by the standard protocol. The expression of BMAL1 mRNA during adipose differentiation in 3T3-L1 cells was determined by Northern blot analysis. TfR, transferrin receptor.