Fig. 2.

The expression of E-selectin is up-regulated by fXa. (A) Time course of E-selectin expression as detected by immunoblotting in HUVECs stimulated by fXa (10 nM), TNF (5 ng/ml), or both. (B) fXa-induced up-regulation of E-selectin or TF requires its proteolytic activity. C921-78 (100 nM), a fXa-specific inhibitor, was added to HUVECs during fXa (10 nM) and TNF (1 ng/ml) stimulation. The expression of E-selectin (Upper) or TF(Lower) was detected by immunoblotting. (C) TF-dependent coagulation assay in the presence of C921-78 (100 nM) during the stimulation of HUVECs by fXa (10 nM) and TNF (1 ng/ml). Representative data from three independent experiments are reported as average ± standard deviation.