Fig. 7.
Inhibition of TNF-induced expression of IκBα and A20 by fXa or PAR agonists, as detected by real-time-PCR. (A) Time course of IκBα expression in HUVECs stimulated for the indicated length of time with fXa (10 nM), TNF (5 ng/ml), or both. (B) IκBα expression in HUVECs incubated for 60 min with 100 μM peptide agonists with or without the addition of TNF (5 ng/ml). (C) Time course of A20 expression in HUVECs stimulated for the indicated length of time with fXa (10 nM), TNF (5 ng/ml), or both. (D) A20 expression in HUVECs incubated with 100 μM peptide agonists with or without the addition of TNF (5 ng/ml) for 60 min. Representative data from at least three independent experiments are reported.