Fig. 3.
Northern and Western blot analyses of total RNA from white adipose tissue and perfused hepatocytes of wild-type and heterozygous mutant Acc1+/–mice. Total RNA from white adipose (A) and hepatocytes (B) of wild-type and mutant Acc+/– mice were subjected to 1% agarose gel in the presence of formalin. The fractionated RNA was transferred to Hybond N (Amersham Pharmacia Biotech). The filters were hybridized with 32P-labeled cDNA of a 362-bp mouse cDNA PCR fragment. To show equal loading, the gel were stained with ethedium bromide to visualize the 28S and 18S rRNA. (C and D) Western blot of liver crude extracts (25 μg) probed with avidin peroxidase and antiphospho-ACC (Ser-79) respectively; lanes: 1, wild type; 2, heterozygous (+/–); M, myosin.