Fig. 1.

Identification of GrIP. (A) Specific GrIP–cdiGRP interaction in the two-hybrid system. The indicated cell inocula were plated on growth media without leucine, tryptophan, and histidine (Left) or without leucine and tryptophan (Right). Growth on histidine-deficient medium represents selective conditions for protein–protein interaction. (B) Detection of in vivo interaction between GrIP and cdiGRP by coimmunoprecipitation with anti-cdiGRP antiserum (lane 1) or with preimmune serum (lane 2). Arrow indicates a ≈16 kDa protein species detected in the immunocomplexes by using Western blotting with anti-GrIP antiserum. (C) Deduced amino acid sequence of GrIP (GenBank accession no. DQ007343).