Fig. 3.

Elevated levels of cdiGRP and callose in GrIP-S1 plants. (A–D) cdiGRP in untreated wild-type plants, wild-type plants treated with 5 μM CdCl₂, untreated GrIP-S1 plants, and GrIP-S1 plants treated with 5 μM CdCl₂, respectively. (E–H) Callose in sections shown in A–D, respectively. (I–L) Merged images from A and E, B and F, C and G, and D and H, respectively. cdiGRP- and callose-specific signals are red and green, respectively; overlapping cdiGRP- and callose-specific signals are yellow. (M–P) Phase contrast images of sections shown in A, E, and I; B, F, and J; C, G, and K; and D, H, and L, respectively. Vascular tissue is seen as smaller cells arranged in bundles and nonvascular tissue is represented by larger cells surrounding the vascular bundles. (Scale bar: 50 μm.)