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. 2025 Feb 25;16:1453344. doi: 10.3389/fimmu.2025.1453344

Table 4.

Potential T-lymphocyte-associated immunotherapies for CCA.

Ref Year Country Experimental methods Tumor type Treatment Outcomes
(102) 2017 China clinical trial CCA CART cells targeting EGFR and CD133 This patient achieved longer progression-free survival with CART-EGFR and CART133 therapy.
(103) 2020 Thailand Cell culture experiment CCA CART cells targeting CD133 Anti-CD133-CAR4T Cell Immunotherapy available to treat patients with CD133-Positive CCA tumor cells
(106) 2021 Thailand Cell culture experiment CCA CART cells targeting MUC1 Anti-MUC1-CAR4 T cells achieve anticancer effects on MUC1-expressing CCA cells by increasing the production of anti-tumor cytokines (TNF-α and IFN-γ), pro-apoptotic proteins (granzyme B), and by inducing lysis of CCA cells.
(107) 2021 Thailand Cell culture experiment CCA CART cells targeting integrin αvβ6 Anti-αvβ6-CAR T cells effectively kill αvβ6-positive CCA cells
(108) 2023 China Cell culture experiment ICC CART cells targeting Tn-MUC1(5E5) Anti-5E5-CAR T cells effectively eliminate Tn-MUC1-positive ICC cells in vitro and in vivo
(110) 2023 Thailand Cell culture experiment CCA aM.CAR/SR T cells: anti-MUC1-CAR (aM.CAR) T cells containing SR molecules (PD-1-CD28) aM.CAR/SR T cells were significantly more cytotoxic to CCA cells expressing MUC1 and PD-L1
(111) 2023 China Cell culture experiment CCA PTG-T16R-scFv-CAR-T cells PTG-T16R-scFV-CAR-T cells knocking down the hexameric inhibitory molecule are highly immune to cholangiocarcinoma cells in vivo and in vitro
(97) 2022 Thailand Cell culture experiment CCA Combination gemcitabine and PD-L1xCD3 bispecific T cell engager (BiTE) BiTE significantly enhanced the cytotoxicity of T lymphocytes against CCA cells, especially after gemcitabine treatment, and the magnitude of cytotoxicity was positively correlated with the expression level of PD-L1
(117) 2023 Thailand Cell culture experimen CCA peptide vaccine Peptide-pulsed DC-activated autologous HLA-A* 11:01-restricted T cells efficiently lysed KKU-213A (HLA-A*11:01) CCA cells compared to conventional tumor lysate-pulsed DC.
(121) 2019 China clinical trial CCA allogenic γδ T cell immunotherapy Allogeneic γδ T cell therapy positively modulated the peripheral blood immune function of the patients, reduced CCA tumor cell activity, and prolonged the life span of the patients.
(122) 2024 Thailand Cell culture experimen CCA Vγ9Vδ2 T cells Vγ9Vδ2 T cells can mediate cytotoxic effects on cholangiocarcinoma