Fig. 4.

Young fibroblast-derived migrasomes alleviate senescence in HaCaT in vitro A. Figure of the purification procedure for young fibroblast-derived migrasomes B. Representative TEM images of negative staining for purified young fibroblast-derived migrasomes C. Representative images of western blot showing the expression of migrasome-related markers in purified young fibroblast-derived migrasomes D. The internalization of migrasomes by senescent HaCaT cells. The migrasomes are specifically labeled with WGA and appear as red puncta, and phalloidin staining in green E. Representative images of SA-β-gal staining in H₂O₂-induced senescent HaCaT cells treated with different concentrations of young fibroblast-derived migrasomes F. Representative Western Blot images showing the expression of senescence markers p16 and p21 in H₂O₂-induced senescent HaCaT cells treated with different concentrations of young fibroblast-derived migrasomes n G. Quantification of SA-β-gal positive cells in H₂O₂-induced senescent HaCaT cells treated with different concentrations of migrasomes for 48 h. Experiments were repeated independently three times. Data were collected by randomly photographing 3 fields per group. Statistical analysis was performed using one-way ANOVA. Error bars indicate the mean ± SEM H and I. Quantitative Western Blot analysis showing the expression of senescence markers p16 and p21 in H₂O₂-induced senescent HaCaT cells after 48 h of stimulation with various concentrations of migrasomes. Experiments were conducted independently three times. Data were analyzed using one-way ANOVA. Error bars indicate the mean ± SEM