FIGURE 5.

13‐AC acted as a potent inhibitor of Hsp 90. (A and B) Molecular modeling of Hsp 90 protein structure with 13‐AC as assessed by Autodock 4.2 software with Lamarckian Genetic Algorithm. (C) The melting temperature shift of 17‐AAG and 13‐AC binding to Hsp 90 protein, as examined with thermal shift assay. The effect of 13‐AC on the expression of Hsp 70 and 90 of Molt4 cells (D) and the Hsp 90 clients of Molt4 cells (E) determined by Western blotting analysis, was a dose‐dependent manner, respectively. Actin was used as the loading control. The statistical differences were denoted at *p < 0.05; **p < 0.01; ***p < 0.001 when compared with the negative control. All experiments were repeated in triplicate.