FIGURE 7.

The apoptotic effect of 13‐AC involved the induction of oxidative stress in Molt4 cells. (A) The apoptosis and (B) disruption of MMP populations were examined with annexin‐V/PI and JC‐1 staining, respectively. The cells were pretreated with 3 mM of NAC for 2 h, then treated with 5 μg/mL of 13‐AC for a further 24 h; (C) Effect of NAC pretreatment on the expression of c‐PARP, Hsp 70, and Topo IIα alone or in a combination of 13‐AC treatment were examined by the Western blotting assay. GAPDH was used as the loading control. The results are presented as means ± SD of three independent experiments (*p < 0.05; **p < 0.01; ***p < 0.001) when compared with the group treated with 13‐AC.