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. 2023 Apr 19;39(5):458–467. doi: 10.1002/kjm2.12676

FIGURE 1.

FIGURE 1

HG induced pyroptosis and the inflammatory response in cardiomyocytes. H9C2 cells were treated with NG (5.5 mM) or HG (30 mM) for 24 h. (A) RT–qPCR was used to evaluate HOTAIR and SIRT3 expression. (B) RT–qPCR was performed to analyze IL‐1β and IL‐18 levels. (C) ELISA was utilized to assess the secretion of IL‐1β and IL‐18. (D) Flow cytometry was conducted to detect pyroptosis. (E) Western blot analysis of pyroptosis‐ and inflammation‐related proteins. The results are expressed as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.