FIGURE 2.

Overexpressed HOTAIR inhibited the HG‐induced pyroptosis and inflammatory response in cardiomyocytes. H9C2 cells were transfected with a HOTAIR overexpression plasmid and treated with HG. (A) The efficiency of HOTAIR overexpression was detected by RT–qPCR. (B) RT–qPCR was used to analyze IL‐1β and IL‐18 levels. (C) ELISA was performed to assess the secretion of IL‐1β and IL‐18. (D) Flow cytometry was utilized to detect pyroptosis. (E) Western blot analysis of pyroptosis‐ and inflammation‐related proteins. The results are expressed as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.