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. 2025 Mar 11;20(3):e0315093. doi: 10.1371/journal.pone.0315093

Fig 1. Phylogenetic tree based on 16S rRNA gene sequences showing the position of Deferrivibrio metallireducens V6Fe1T in the family Deferrivibrionaceae, related to the families Deferribacteraceae and Geovibrionaceae.

Fig 1

The outgroup (not represented here) was composed of the following type species: Thermanaeromonas toyohensis ToBE, Moorella thermoacetica DSM 2955, Moorella humiferrea OCP, Moorella glycerini DSM 11254, Desulfonatronum thiosulfatophilum ASO4-2, Desulfonatronum thioautotrophicum ASO4-1, Desulfonatronum thiodismutans MLF1, Desulfonatronum lacustre DSM 10312, Desulfovibrio ferrophilus IS5, Desulfovibrio subterraneus ND17, Desulfovibrio mangrove FT415, Desulfovibrio desulfuricans L4, Desulfovibrio fairfieldensis CCUG 45958, Candidatus Desulfovibrio trichonymphae Rs-N31. 16S rRNA gene sequences were extracted from genome assemblies using barrnap 0.9, and the multiple alignments of the sequences were performed with mafft 7.515 [39]. The maximum-likelihood phylogenetic tree was generated using IQ-TREE 2.3.4 [40] with GTR + F + G4 model. Bootstraps are depicted at nodes, estimated by IQ-TREE -B 1000 option. The tree was generated using iTOL v6, providing a visual representation of the phylogenetic connections. The scale bar indicates the number of substitutions per site.