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. 2025 Feb;18(2):7–15.

TABLE 1.

The Stratum Corneum and Simultaneous Multitasking: Individual Epidermal Barrier Functions

BARRIER FUNCTION MAJOR COMPONENTS
Permeability Barrier Formation of stratum corneum lipids in specific ratio from precursor lipids
Production of lamellar bodies packaging precursor lipids and some antimicrobial peptides
Formation of natural moisturizing factor from filaggrin (converted from profillagrin)
Formation of cornified envelope and the corneocyte-lipid envelope
Maintenance of water gradient, calcium gradient, acid mantle (acidic pH)
Response of primary pro-inflammatory cytokines to impairment of permeability barrier
Antimicrobial Barrier Maintenance of an acidic skin pH decreases skin colonization by pathogenic bacteria
Antibacterial activity of stratum corneum lipids (eg, free fatty acids, sphingosine, other)
Genetically-encoded primary antimicrobial peptides (defensins, cathelicidins, dermcidins) synthesized in SC, present in sebum, and in sweat (dermicidin-derived)
Multiple agents with antimicrobial activity as alternative function (some chemokines, some neuropeptides, other)
Antioxidant Barrier Network of enzymatic and non-enzymatic antioxidant systems to counter oxidative stress
Antioxidants present in epidermis (stratum corneum, skin surface lipids) and dermis
Hydrophilic non-enzymatic antioxidants include ascorbic acid (vitamin C) and uric acid
Major lipid-soluble non-enzymatic antioxidant is alpha-tocopherol (vitamin E)
Co-antioxidants (ascorbic acid, ubiquinol [Coenzyme Q10]) allow tocopherol regeneration
Gradients in stratum corneum for ascorbic acid and tocopherol (lowest near surface)
Interceptive antioxidant enzymes (catalase, superoxide dismutases, glutathione peroxidases)
Antioxidant repair enzymes (eg, methionine sulfoxide reductase)
High concentration of alpha-tocopherol in sebum accounts for high levels in facial sebaceous gland stratum corneum (sebum serves as a physiologic delivery pathway)
Immune Response Barrier Dendritic cells involved in immune surveillance and antigen recognition (eg, plasmacytoid dendritic cells, myeloid dendritic cells, Langerhans cells)
Toll-like receptors involved in recognition of microbial pathogens and other agonists
Antimicrobial peptides and some of their enzymatic conversion products (eg, LL-37)
Innate and acquired immune response pathways and balance with T regulatory cell system
Photoprotection Barrier Epidermal melanin barrier (degree of protection related to Fitzpatrick Skin Type)
Stratum corneum protein barrier
Antioxidants within stratum corneum (protection against photo-oxidative stress)
Optical reflective properties of the stratum corneum (stratum corneum thickness more important than epidermal thickness for protection against ultraviolet/solar radiation)