Skip to main content
PMC home page
Canadian Journal of Veterinary Research logoLink to Canadian Journal of Veterinary Research
. 2001 Oct;65(4):223–228.

Effects of exogenous recombinant interleukin-12 on immune responses and protection against Brucella abortus in a murine model.

I K Lee 1, S C Olsen 1, C A Bolin 1
PMCID: PMC1189683  PMID: 11768128

Abstract

This study determined if murine interleukin-12 (IL-12) would influence immunity in mice vaccinated with live or killed Brucella abortus strain RB51 (SRB51). Mice received live or gamma-irradiated SRB51 bacteria alone, or with IL-12 (0.5 or 1.0 microg, 2x or 3x), whereas other mice received saline or IL-12 alone. Post-vaccination antibody responses to live or killed SRB51 and clearance of live SRB51 from splenic tissue were not influenced by IL-12 treatments. Mice were challenged at 12 weeks with 4 x 10(4) cfu of B. abortus strain 2308 (S2308) and were euthanized 2 weeks later. The highest IL-12 treatment increased (P < 0.05) post-challenge antibody responses when co-administered with killed SRB51. Co-administration of 1.0 microg of IL-12 with live SRB51, but not killed SRB51, reduced (P < 0.05) S2308 colonization of splenic tissues. Our data suggest that although IL-12 may augment protective immunity induced by live SRB51, it does not influence protection induced by vaccination with killed SRB51.

Full text

PDF
224

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Afonso L. C., Scharton T. M., Vieira L. Q., Wysocka M., Trinchieri G., Scott P. The adjuvant effect of interleukin-12 in a vaccine against Leishmania major. Science. 1994 Jan 14;263(5144):235–237. doi: 10.1126/science.7904381. [DOI] [PubMed] [Google Scholar]
  2. Bliss J., Van Cleave V., Murray K., Wiencis A., Ketchum M., Maylor R., Haire T., Resmini C., Abbas A. K., Wolf S. F. IL-12, as an adjuvant, promotes a T helper 1 cell, but does not suppress a T helper 2 cell recall response. J Immunol. 1996 Feb 1;156(3):887–894. [PubMed] [Google Scholar]
  3. Brunda M. J. Interleukin-12. J Leukoc Biol. 1994 Feb;55(2):280–288. doi: 10.1002/jlb.55.2.280. [DOI] [PubMed] [Google Scholar]
  4. Cheville N. F., Stevens M. G., Jensen A. E., Tatum F. M., Halling S. M. Immune responses and protection against infection and abortion in cattle experimentally vaccinated with mutant strains of Brucella abortus. Am J Vet Res. 1993 Oct;54(10):1591–1597. [PubMed] [Google Scholar]
  5. Fernández-Lago L., Monte M., Chordi A. Endogenous gamma interferon and interleukin-10 in Brucella abortus 2308 infection in mice. FEMS Immunol Med Microbiol. 1996 Sep;15(2-3):109–114. doi: 10.1111/j.1574-695X.1996.tb00060.x. [DOI] [PubMed] [Google Scholar]
  6. Fernández-Lago L., Rodríguez-Tarazona E., Vizcaíno N. Differential secretion of interleukin-12 (IL-12) subunits and heterodimeric IL-12p70 protein by CD-1 mice and murine macrophages in response to intracellular infection by Brucella abortus. J Med Microbiol. 1999 Dec;48(12):1065–1073. doi: 10.1099/00222615-48-12-1065. [DOI] [PubMed] [Google Scholar]
  7. Ficht T. A., Bearden S. W., Sowa B. A., Adams L. G. DNA sequence and expression of the 36-kilodalton outer membrane protein gene of Brucella abortus. Infect Immun. 1989 Nov;57(11):3281–3291. doi: 10.1128/iai.57.11.3281-3291.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Germann T., Bongartz M., Dlugonska H., Hess H., Schmitt E., Kolbe L., Kölsch E., Podlaski F. J., Gately M. K., Rüde E. Interleukin-12 profoundly up-regulates the synthesis of antigen-specific complement-fixing IgG2a, IgG2b and IgG3 antibody subclasses in vivo. Eur J Immunol. 1995 Mar;25(3):823–829. doi: 10.1002/eji.1830250329. [DOI] [PubMed] [Google Scholar]
  9. Jiménez de Bagüs M. P., Elzer P. H., Jones S. M., Blasco J. M., Enright F. M., Schurig G. G., Winter A. J. Vaccination with Brucella abortus rough mutant RB51 protects BALB/c mice against virulent strains of Brucella abortus, Brucella melitensis, and Brucella ovis. Infect Immun. 1994 Nov;62(11):4990–4996. doi: 10.1128/iai.62.11.4990-4996.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lee I. K., Olsen S. C., Kehrli M., Bolin C. A. The adjuvant effect of a single dose of interleukin-12 on murine immune responses to live or killed Brucella abortus strain RB51. Can J Vet Res. 1999 Oct;63(4):284–287. [PMC free article] [PubMed] [Google Scholar]
  11. Manetti R., Parronchi P., Giudizi M. G., Piccinni M. P., Maggi E., Trinchieri G., Romagnani S. Natural killer cell stimulatory factor (interleukin 12 [IL-12]) induces T helper type 1 (Th1)-specific immune responses and inhibits the development of IL-4-producing Th cells. J Exp Med. 1993 Apr 1;177(4):1199–1204. doi: 10.1084/jem.177.4.1199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McKnight A. J., Zimmer G. J., Fogelman I., Wolf S. F., Abbas A. K. Effects of IL-12 on helper T cell-dependent immune responses in vivo. J Immunol. 1994 Mar 1;152(5):2172–2179. [PubMed] [Google Scholar]
  13. Miller M. A., Skeen M. J., Ziegler H. K. Protective immunity to Listeria monocytogenes elicited by immunization with heat-killed Listeria and IL-12. Potential mechanism of IL-12 adjuvanticity. Ann N Y Acad Sci. 1996 Oct 25;797:207–227. doi: 10.1111/j.1749-6632.1996.tb52962.x. [DOI] [PubMed] [Google Scholar]
  14. Schurig G. G., Roop R. M., 2nd, Bagchi T., Boyle S., Buhrman D., Sriranganathan N. Biological properties of RB51; a stable rough strain of Brucella abortus. Vet Microbiol. 1991 Jul;28(2):171–188. doi: 10.1016/0378-1135(91)90091-s. [DOI] [PubMed] [Google Scholar]
  15. Stevens M. G., Olsen S. C., Pugh G. W., Jr, Brees D. Comparison of immune responses and resistance to brucellosis in mice vaccinated with Brucella abortus 19 or RB51. Infect Immun. 1995 Jan;63(1):264–270. doi: 10.1128/iai.63.1.264-270.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stevens M. G., Pugh G. W., Jr, Tabatabai L. B. Effects of gamma interferon and indomethacin in preventing Brucella abortus infections in mice. Infect Immun. 1992 Oct;60(10):4407–4409. doi: 10.1128/iai.60.10.4407-4409.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Stevens T. L., Bossie A., Sanders V. M., Fernandez-Botran R., Coffman R. L., Mosmann T. R., Vitetta E. S. Regulation of antibody isotype secretion by subsets of antigen-specific helper T cells. Nature. 1988 Jul 21;334(6179):255–258. doi: 10.1038/334255a0. [DOI] [PubMed] [Google Scholar]
  18. Zhan Y., Cheers C. Endogenous gamma interferon mediates resistance to Brucella abortus infection. Infect Immun. 1993 Nov;61(11):4899–4901. doi: 10.1128/iai.61.11.4899-4901.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Zhan Y., Cheers C. Endogenous interleukin-12 is involved in resistance to Brucella abortus infection. Infect Immun. 1995 Apr;63(4):1387–1390. doi: 10.1128/iai.63.4.1387-1390.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Zhan Y., Kelso A., Cheers C. Cytokine production in the murine response to brucella infection or immunization with antigenic extracts. Immunology. 1993 Nov;80(3):458–464. [PMC free article] [PubMed] [Google Scholar]
  21. Zhan Y., Liu Z., Cheers C. Tumor necrosis factor alpha and interleukin-12 contribute to resistance to the intracellular bacterium Brucella abortus by different mechanisms. Infect Immun. 1996 Jul;64(7):2782–2786. doi: 10.1128/iai.64.7.2782-2786.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Canadian Journal of Veterinary Research are provided here courtesy of Canadian Veterinary Medical Association

RESOURCES