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. 2025 Mar 11;22(3):e70145. doi: 10.1111/iwj.70145

FIGURE 2.

FIGURE 2

Enzymatic debridement agents dispersed biofilms in wound tissue, ex vivo. The wound beds from mice infected for 48 h with PAO1 were surgically extracted and were suspended in enzymatic treatments for 3 h. Percent dispersal was calculated as follows: [(CFU/mL dispersed fraction)/((CFU/mL dispersed fraction) + (CFU/mL remaining biofilm))] * 100%. (A) GH dispersal was dose‐dependent but remained effective at lower doses. (B) Papain dispersal was dose‐independent. (C) Collagenase dispersal was dose‐dependent (D) at a standardised enzymatic activity level (3 U/mL), GHs, papain (PAP), bromelain (BRO), and collagenase (COL) dispersed biofilm with varying efficacy. Data were analysed with a one‐way ANOVA and Mann–Whitney tests. *p < 0.05, **p < 0.01 compared to the PBS vehicle control.