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. 2025 Mar 12;10:84. doi: 10.1038/s41392-025-02166-2

Table 4.

Preclinical studies of targeting HSP70

Compound Structure Activity Mechanism (Sites)
VER-155008 graphic file with name 41392_2025_2166_Tabaw_HTML.gif bIC50 = 0.50 μM Pan-HSP70 inhibitor (NBD)
methylene blue graphic file with name 41392_2025_2166_Tabax_HTML.gif / Pan-HSP70 inhibitor (NBD)
YK-5 graphic file with name 41392_2025_2166_Tabay_HTML.gif cIC50 = 0.80 μM (SkBr3 cells) Pan-HSP70 inhibitor (NBD)
PES graphic file with name 41392_2025_2166_Tabaz_HTML.gif / Pan-HSP70 inhibitor (SBD)
AEAC graphic file with name 41392_2025_2166_Tabba_HTML.gif Kd = 149 nM Pan-HSP70 inhibitor (SBD)
Apoptozole graphic file with name 41392_2025_2166_Tabbb_HTML.gif

HSP70i: Kd = 0.14 μM;

HSC70: Kd = 0.21 μM

 HSP70i/HSC70 selective inhibitor (NBD)
HS-72 graphic file with name 41392_2025_2166_Tabbc_HTML.gif / HSP70i selective inhibitor (NBD)
Pinaverium bromide graphic file with name 41392_2025_2166_Tabbd_HTML.gif cIC50 = 10 μM (A2058 cells) HSC70 selective inhibitor (NBD)
EGCG graphic file with name 41392_2025_2166_Tabbe_HTML.gif Kd = 0.70 μM GRP78 selective inhibitor (NBD)
HA15 graphic file with name 41392_2025_2166_Tabbf_HTML.gif aIC50 = 25–50 μM GRP78 selective inhibitor (NBD)
YUM70 graphic file with name 41392_2025_2166_Tabbg_HTML.gif aIC50 = 1.5 μM GRP78 selective inhibitor (SBD)
Hexachlorophene graphic file with name 41392_2025_2166_Tabbh_HTML.gif dIC50 = 9.1 μM GRP78 selective inhibitor (SBD)
TPP-apoptozole graphic file with name 41392_2025_2166_Tabbi_HTML.gif / GRP75 selective inhibitor (SBD)
MAL3-101 graphic file with name 41392_2025_2166_Tabbj_HTML.gif / HSP70-HSP40 PPI inhibitor (NBD)
S1g-2 graphic file with name 41392_2025_2166_Tabbk_HTML.gif

eIC50 = 0.40 μM;

Kd = 0.84 μM

 HSP70-BIM PPI inhibitor (NBD)
YM-1 graphic file with name 41392_2025_2166_Tabbl_HTML.gif

eIC50 = 4.8 μM;

Kd = 5.8 μM

 HSP70-BAGs PPI inhibitor (NBD)
JG-98 graphic file with name 41392_2025_2166_Tabbm_HTML.gif

eIC50 = 1.6 μM;

Kd = 86 nM

 HSP70-BAGs PPI inhibitor (NBD)
SY7 graphic file with name 41392_2025_2166_Tabbn_HTML.gif / HSP70-HOP PPI inhibitor (SBD)
SY8 graphic file with name 41392_2025_2166_Tabbo_HTML.gif / HSP70-HOP PPI inhibitor (SBD)

Kd target binding affinity; Ki target binding affinity; aIC50 ATPase inhibitor activity; bIC50 competitive binding activity; cIC50 anti-proliferative activity; dIC50 substrate competitive inhibitory activity; eIC50 PPIs inhibitory activity; “/” indicates no clear reports