FIGURE 2.

Prolactin increases β-tubulin III-positive cells in early differentiation, primarily at 6 nM. (A) Representative images of β-tubulin III (green)/DAPI (blue) positive cells in control and PRL-treated groups, across concentrations from 0.2 to 20 nM. Images were acquired using identical gain and exposure settings on an epifluorescence microscope. Scale bar: 25 μm. (B) Quantification of β-Tubullin III-positive (immature neurons) cells at day 12 of differentiation. PRL treatment increased β-Tubullin III-positive cells at all concentrations, with 6 nM yielding significant statistical difference. Bar plot, mean ± SEM. Tukey´s multiple comparisons test, p < 0.05. F, DFn, Dfd values: 2.854, 5 and 18. Data represent three replicates analyzed in duplicate.