Table 3.
Sequence of primers for microbial sensing and intestinal integrity in the jejunum of nursery pigs fed diets supplemented with ground herb-based phytobiotics1 and essential oil-based phytobiotics2
| Gene3 | Primer sequences (5′–3′)4 | Accession number | Size |
|---|---|---|---|
| NOD1 | F: AACACCGATCCAGTGAGCAG R: AAATGGTCTCGCCCTCCTTG |
NM_001114277.1 | 230 |
| NOD2 | F: GTGCCTCCCCTCTAGACTCA R: ACGAACCAGGAAGCCAAGAG |
NM_001105295.1 | 191 |
| TLR2 | F: GGGCTGCGTTCATTCATCAG R: CTGCAGAGGATGGATGGCAA |
XM_005653576.3 | 132 |
| TLR4 | F: CGTGCAGGTGGTTCCTAACA R: GGTTTGTCTCAACGGCAACC |
NM_001113039.2 | 326 |
| CLD | F: AAACCGTGTGGGAACAACCA R: CACATGAAAATGGCTTCCCTC |
NM_001244539.1 | 196 |
| OCC | F: TCAGGTGCACCCTCCAGATT R: AGGAGGTGGACTTTCAAGAGG |
XP_005672579.1 | 169 |
| ZO-1 | F: CAGAGACCAAGAGCCGTCC R: TGCTTCAAGACATGGTTGGC |
XM_003480423.4 | 105 |
1Salcochek Pro (Ayurvet Limited).
2Liq-biotic (Ayurvet Limited).
3NOD1, nod-like receptor 1; NOD2, nod-like receptor 2; TLR2, toll-like receptor 2; TLR4, toll-like receptor 4; CLD, claudin; OCC, occludin; ZO-1, zonula occludens-1.
4F, forward; R, reverse.