Table 1.
Bacterial strains, Plasmids, and primers used in this study.
| Bacteria and plasmids | Description | Reference or Source |
|---|---|---|
| Bacteria | ||
| K. pneumoniae | ||
| NTUH-K2044 (WT) | Wild-type strain; Apr; parent strain for generation of isogenic mutant strains | [23] |
| ΔmodA | Deletion of modA from WT; Apr | Present study |
| C-modA | Complemented modA mutant; Apr; Kmr | Present study |
| ΔnarG | Deletion of narG from WT; Apr | Present study |
| ΔnarZ | Deletion of narZ from WT; Apr | Present study |
| ΔnarGΔnarZ | Deletion of narG,narZ from WT; Apr | Present study |
| Plasmids | ||
| pKO3-Km | pKO3-derived plasmid, with an insertion of Km resistance cassette from pUC4K into AccI site | [25] |
| pGEM-T-easy-km | pGEM-T easy with an insert of Km cassette from pUC4K into NdeI site | [26] |
| Primers | ||
| modA Mutant constructs | ||
| modA-A | GTATGCGGCCGCATCCACCAACGGATGTTCGC | |
| modA-B | GCCCTTTCAGGTAGTCATAGCTTACTCTCCTGTCAATGCG | |
| modA-C | CGCATTGACAGGAGAGTAAGCTATGACTACCTGAAAGCGC | |
| modA-D | GTATGCGGCCGCCAACGGCAACGTAATGGTC | |
| modA-F | ATGGCAGGTTCTTGGTTACG | |
| modA-R | ACTTTCTGATGCGAGGCTTC | |
| Complemented modA mutant | ||
| C-modA-F | ATGGGCCCGAGATGGCCGCCAGCAGG | |
| C-modA-R | TAAAGCGGCCGCTCAGCGGGTGGTAAATCCGT | |
| narG Mutant constructs | ||
| narG-A | GTATGCGGCCGCTTCGGTATCTCGCTGGAT | |
| narG-B | TTACGCTCTCCTGTACCTGGCGGGTTTCTCCTGATATGG | |
| narG-C | CCATATCAGGAGAAACCCGCCAGGTACAGGAGAGCGTAA | |
| narG-D | GTATGCGGCCGCAGGATCTCTTCCCAGTTCG | |
| narG-F | ATGAAGATGTGGCGGGAAG | |
| narG-R | GCAGAGTTTGGCGATTTCG | |
| narZ Mutant constructs | ||
| narZ-A | GTATGCGGCCGCCTCCACCTTCCAGATGATCG | |
| narZ-B | GTACCTGATCGCGACCTTGACATTTCTCCTGCTCCGAT | |
| narZ-C | ATCGGAGCAGGAGAAATGTCAAGGTCGCGATCAGGTAC | |
| narZ-D | GTATGCGGCCGCCCATCTTCCTCACGCTTGTAGA | |
| narZ-F | CCAGCAGTTGTATCAGGACC | |
| narZ-R | ACCGAGTTATGAATGCCG | |