Figure 1.

Phase transition in full-tube coverage as a function of SNP-SNP compatibility probability. These results are based on a simulations where the controlling parameter P denotes the probability that two SNPs are compatible. Two SNPs are compatible if their associated primers are all pair-wise compatible. This simulation is based on N = 1,200 SNPS and S = 500 primer pairs per SNP. In reality, this compatibility probability, P, depends on the stringency by which primer pairs are tested for cross-interactions. As we increase the target multiplexing level, higher compatibility, beyond what are normally obtained using standard primer selection criteria is required, suggesting fundamental barriers to increasing target multiplexing levels.