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. 2005 Aug 4;5:13. doi: 10.1186/1471-2229-5-13

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Copyright © 2005 Reisen et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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PEG-stressed cells. (a, b) Localization of BobTIP26-1::GFP in the membrane of spherical structures (arrowheads) within vacuoles. Bar = 10 μm. (c) A histogram of fluorescent intensity values collected from both the tonoplast of the cell periphery and vesicles. (d) A 3-D slice reconstruction through the vacuole at the position of the nucleus (n). Arrowhead = spherical structure. (e) 3-D reconstruction through a part of a vacuole. Transvacuolar strands are still present in these cells (arrow); spherical structures are primarily fixed onto the tonoplast (arrowheads).