Figure 7.

Metastasis of GFP-expressing human breast MDA MB231 cells from the site of inoculation in the inguinal mammary fat pad to lodge in the lungs of mice in each of the four treatment groups. The lungs were removed and smashed between microscopic slides and microcolonies of GFP cells were observed by epilumination using blue light and detected by presence of green fluorescence microcolonies observed using both 3.5× and 10× objective lens. The untreated mice had a significantly higher number of GFP positive microcolonies than the groups of mice that received gamma irradiation therapy or those mice that received EMF therapy. There were no other significant differences between groups in either mean number of microcolonies per lung or in incidence. Horizontal lines indicate mean values for each treatment group.