FIG. 6.
YPK2D239A acts as a tor2-specific suppressor. A. (Top) YPK2 alleles (cloned into YEp352) harboring mutations at D239 or/and T501 (Pkh phosphorylation site) were transformed into YYK373 (ypksΔ p415GAL1[YPK2-224]). The transformants were grown on YEPgalactose (Ypk2-224 expressed; left) or YEPD (Ypk2-224 not expressed; center) plates for 2 days at 30°C. (Bottom) A temperature-sensitive tor2-21 mutant (SH121 [TOR1 tor2-21]) was transformed with YEp352-based YPK2 plasmids as indicated (right) and grown on YEPD for 2 days at 30°C (left) or 36.5°C (center). Wild-type control cells (SH100 [TOR1 TOR2]) are also shown. B. Temperature-sensitive pkh mutant cells (INA106-3B [pkh1D398G pkh2Δ]) and wild-type control cells (INA28-1B [PKH1 pkh2Δ]) were transformed with YEp352-based YPK2 plasmids as indicated (right) and grown on YEPD for 2 days at 30°C (left) or 36.5°C (center).