FIG. 5.

TCR internalization in HIP-55 knockout T cells. (A) Splenocytes from wild-type (WT) and HIP-55 knockout (KO) mice were unstimulated or stimulated with biotin-anti-CD3ɛ (10 μg/ml) for 2 h at 37°C and then stained with fluorescein isothiocyanate-labeled anti-CD4 antibody and Tricolor-labeled streptavidin. The mean fluorescence intensity in the lower panel represents the intensity of CD3ɛ in the CD4⁺ population. Data are derived from three mice in each group. (B) Splenocytes from wild-type (WT) and HIP-55 knockout (KO) mice were unstimulated or stimulated with prebound anti-CD3ɛ (10 μg/ml) for 2 or 4 h at 37°C and then stained with fluorescein isothiocyanate-labeled anti-CD4 and phycoerythrin-labeled anti-TCRβ antibodies. The mean fluorescence intensity represents the intensity of TCRβ in the CD4⁺ population. Data are derived from three mice in each group. (C) HIP-55 shRNA and vector-transfected Jurkat T cells were stained with an anti-CD3ɛ antibody. Negative stands for no staining (negative control).