FIG. 6.

Reduced antigen-specific antibody production in HIP-55 knockout mice. (A) Five wild-type (WT) and five HIP-55 knockout (KO) mice were immunized with NP-CGG. Serum was collected at 7 and 30 days (day 0 for secondary response) after the first immunization and 5 days after the second immunization. NP-specific IgG1 production was determined by ELISA and is presented as relative units compared to the standard. Statistical analysis was performed using the Student t test. The data are derived from five mice in each group; the difference is statistically significant (P < 0.02 for day 7, P < 0.01 for day 30 and day 5 after the boost). (B) Anti-NP-specific IgM from immunized mice was measured as described for A.