FIG. 3.

Expression of other urea transporters. A. Immunofluorescence of UT-A1 and UT-A3 in the inner medulla. Antibodies #47 and #57 recognized UT-A1 and UT-A3, respectively, in the collecting ducts of the inner medulla. Their density and cellular localization did not appear to change in UT-A2 knockout mice. Bar = 20 μm. B. Immunoblots of the inner medulla with antibodies #47 and #57. Antibodies #47 and #57 recognized UT-A1 bands of ∼100 and 120 kDa (6), and UT-A3 bands of ∼40 and 50 kDa (6), respectively, in inner medulla of wild-type and UT-A2^−/− mice. All of these bands were absent following preincubation of the antibodies with the relevant immunizing peptides. C. Real-time RT-PCR analysis of renal urea transporters. Total RNA from whole mouse kidney was used as a starting material. UT-B expression was significantly increased in UT-A2^−/− mice. P < 0.05, n = 5.