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. 2005 Aug;25(16):7033–7041. doi: 10.1128/MCB.25.16.7033-7041.2005

FIG. 2.

FIG. 2.

Effects of the HS3 mutants on formation of DNase I hypersensitive sties. The βYAC constructs are labeled on the upper part on each panel. (A) Detection of HSs 1 and 2. DNA was digested with restriction enzyme NdeI; the blot was hybridized with a 3′ probe. The parental band is 8.7 kb; subbands are 2.1 kb (HS1) and 6.9 kb (HS2). (B) Detection of HSs 4 and 5. DNA was digested with restriction enzyme ScaI; the blot was hybridized with a 5′ probe. The parental band is 11.5 kb; subbands are 4 kb (HS5) and 6.4 kb (HS4). (C) Detection of HSs 6 and 7. DNA was digested with restriction enzyme ScaI; the blot was hybridized with a 3′ probe. The parental band is 12 kb; subbands are 2.4 and 2.5 kb (HS6) and 9 kb (HS7). (D) Detection of 3′HS1. DNA was digested with restriction enzyme ScaI; the blot was hybridized with a 3′ probe. The parental band is 9 kb; the subband is 2.5 kb.