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. 2005 Aug;25(16):6879–6888. doi: 10.1128/MCB.25.16.6879-6888.2005

FIG. 2.

FIG. 2.

Expression of mouse histone H2a mRNA in HeLa cells. (A) Mouse histone mRNA is properly regulated in HeLa cells. Five micrograms of total RNA from untreated cells (lane 1) or cells that were treated with either HU for 45 min (lane 2) or CH for 45 min (lane 3) was analyzed by an S1 nuclease assay using the WT mouse histone H2a gene, which detects the 5′ end of histone H2a mRNAs, as a probe. The protected fragments were resolved by gel electrophoresis and detected by radioautography. The protected fragments are 192 nt for WT mouse histone H2a mRNA (mH2a) and 149 nt for the endogenous human H2a mRNA (hH2a). (B) Effects of DF and HEM treatments on histone mRNA. Five micrograms of total RNA from untreated cells (lane 1) or cells that were treated with either 5 mM DF for 2 (lane 2), 3 (lane 3), or 4 h (lane 4) or 5 mM HEM for 4 h (lane 5) was analyzed by an S1 nuclease assay using a mixture of the human replication-dependent histone H2a (hH2a) and replacement-type histone H3.3 (hH3.3) genes as a probe. The protected fragments are 198 nt for histone H2a mRNA and 411 nt for H3.3 mRNA.

HHS Vulnerability Disclosure