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. 2005 Aug;25(16):6879–6888. doi: 10.1128/MCB.25.16.6879-6888.2005

FIG. 3.

FIG. 3.

Regulation of the histone mRNAs containing an IRE in the 5′ UTR. Five micrograms of total RNA from HeLa cells stably expressing the H2a-IREMUT gene (A) or the H2a-IREWT gene (B) was analyzed by an S1 nuclease assay using the H2a-IREMUT gene (A) or the H2a-IREWT gene (B) as a probe. The cells were untreated cells (lanes 1 to 3) or cells treated for 2 h with either DF (lanes 4 to 6) or HEM (lanes 7 to 9). After the initial treatment, these cells were either not treated (lanes 1, 4, and 7) or treated for an additional 45 min with either HU (lanes 2, 5, and 8) or CH (lanes 3, 6, and 9).

HHS Vulnerability Disclosure