FIG. 1.
Composition of tSNAPc and its associated TBP. (A) Protein fractions from IgG chromatography (lane 1), calmodulin flowthrough (lane 2), calmodulin wash (lane 3), EGTA elution (lane 4), and markers (BenchMark; Invitrogen) (lane 5) are shown on an SDS-10% polyacrylamide gel. The three subunits of tSNAPc (tSNAPP50, tSNAP42, and tSNAP26) and TBP, as well as p63 and TFIIA-γ, are identified by arrows. (B) Purification scheme of TAP-tSNAPc and TBP. (C) Silver-stained SDS-10% polyacrylamide gel of 5 to 20% sucrose gradient fractions with peak transcription factor activity.