FIG. 3.

Multiple sequence alignment of T. brucei (Tb) SNAP42 with its orthologs from T. cruzi (Tc), L. major (Lm), and L. seymouri (Ls) using CLUSTALW. Dark shading indicates identical or conserved amino acids in all five proteins. Medium shading with white letter overlays shows amino acid similarity among four proteins. Medium shading alone indicates amino acid similarity between three sequences. The signature triplet helices, present in a single Myb domain, are overlined. The Escherichia coli MutH (P06722) alignment shown was obtained by ProFit analysis. The three active site amino acids (D, E, and K) in MutH that aligned in structure and sequence to the trypanosome proteins are underlined.