TABLE 3.
Quantitative behavioral analysis of Nxph3 knockout micea
| Behavioral test | Result for genotype indicated (no. of animals tested)
|
P | |
|---|---|---|---|
| Wild type | Nxph3 KO | ||
| Grip strength (pond) | 114.8 ± 6.3 (10) | 118.4 ± 5.9 (9) | NS |
| Open field | |||
| Walking speed (cm/s) | 6.5 ± 0.8 (6) | 4.9 ± 0.8 (10) | NS |
| Total distance (m) | 56.4 ± 6.5 (6) | 42.5 ± 6.6 (10) | NS |
| Time in outfield (% of time) | 83.7 ± 3.9 (6) | 69.4 ± 6.6 (10) | NS |
| Time in center (% of time) | 16.3 ± 3.8 (6) | 30.2 ± 6.9 (10) | NS |
| Time in corners (% of time) | 44.2 ± 2.6 (6) | 36.7 ± 6.5 (10) | NS |
| Light-dark avoidance | |||
| Light compartment (% of time) | 75.6 ± 9.0 (6) | 68.4 ± 7.6 (10) | NS |
| Dark compartment (% of time) | 24.4 ± 9.0 (6) | 31.6 ± 7.6 (10) | NS |
| Compartment changes (no.) | 26.7 ± 11.8 (6) | 23.6 ± 4.6 (10) | NS |
| Two-way active avoidance (% active avoidance reactions) | |||
| Day 1 | 7.0 ± 1.1 (10) | 5.4 ± 1.8 (9) | NS |
| Day 2 | 30.6 ± 6.4 (10) | 17.6 ± 4.3 (9) | NS |
| Day 3 | 59.5 ± 7.9 (10) | 45.5 ± 9.4 (9) | NS |
| Day 4 | 67.4 ± 7.1 (10) | 61.5 ± 10.8 (9) | NS |
| Day 5 | 79.8 ± 3.9 (10) | 72.1 ± 8.3 (9) | NS |
| Morris water maze | |||
| Swim path length (m) | |||
| Day 1 (acquisition) | 11.6 ± 0.9 (11) | 10.7 ± 1.0 (13) | NS |
| Day 2 (acquisition) | 9.1 ± 0.9 (11) | 7.1 ± 0.9 (13) | NS |
| Day 3 (acquisition) | 7.6 ± 0.8 (11) | 6.9 ± 0.8 (13) | NS |
| Day 4 (reversal) | 7.2 ± 0.9 (11) | 7.9 ± 0.8 (13) | NS |
| Day 5 (reversal) | 7.5 ± 0.9 (11) | 7.6 ± 0.8 (13) | NS |
| Escape latency (s) | |||
| Day 1 (acquisition) | 73.4 ± 5.9 (11) | 55.8 ± 5.3 (13) | 0.028 |
| Day 2 (acquisition) | 62.1 ± 6.0 (11) | 34.6 ± 4.2 (13) | 0.0002 |
| Day 3 (acquisition) | 49.3 ± 4.8 (11) | 32.6 ± 3.5 (13) | 0.0048 |
| Day 4 (reversal) | 51.2 ± 5.5 (11) | 39.9 ± 4.0 (13) | NS |
| Day 5 (reversal) | 53.1 ± 5.2 (11) | 39.2 ± 3.7 (13) | 0.027 |
a
Data are means ± SEM. All animals used in the behavioral analysis were littermates from heterozygous parents in a mixed 129SvJ × C57BL/6 × FVB background. Exact conditions of tests are described in Materials and Methods. NS, not significant.