FIG. 4.

Examination of precipitated proteins for known proline-based WW domain-binding motifs. The proteins precipitated with each of the GST-WW pulldowns were analyzed for the presence of the indicated proline-containing motifs. Note that the pie charts do not equal 100%, since individual proteins can have more than one motif. The number of proteins precipitated by each WW domain and the percentage of proteins with at least one of the proline-rich motifs are indicated below the corresponding pie charts. The ∼24,000 reference proteins with unique GeneIDs were downloaded from the National Center for Biotechnology Information database and searched locally for the indicated motifs.