FIG. 7.

Protein complexes precipitated by GST-WW domains. (A) The transcriptional complex precipitated by the AIP4 WW2 domain is shown. The WW domain is indicated in yellow, direct WW domain-binding proteins in red, and likely indirectly precipitating proteins in green. Gene symbols and common names (in parentheses) are provided. Black lines indicate a direct physical interaction, while gray lines indicate a physical association, but not necessarily direct. (B) Actin cytoskeleton-regulating complex precipitated by the FE65 WW domain (as well as other group B [II/III] WW domains) is shown and labeled as described above for panel A. Although not found in the FE65 pulldowns, NAP1 (NCKAP1) was included (in blue) as it has been shown to bridge ABI1 with CYFIP1/2 (24, 35) and was found in association with other group B (II/III) WW domains (Fig. 3). Note that this model represents one possible cytoskeleton-regulating complex based on published work. Since the WASP proteins (WAS and WASL), WIPIP, WIRE, WASF2, and ABI1 are extremely proline rich and contain motifs that are predicted to interact with group B WW domains, they may interact directly with group B WW domains.