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Effects of F on DNA synthesis by hPolκ. (A) dTTP, dFTP, or dATP incorporation opposite template A. (B) Incorporation of dATP opposite a template T or F. (C) Rates of dATP incorporation opposite a template T (left) and a template F (right), graphed as a function of [dATP]. The solid line represents the best fit to the Michaelis-Menten equation. Steady-state kinetic parameters are listed in Table 1. A portion of the DNA substrates used is shown in panel B.
