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. 2005 Aug;25(16):7181–7192. doi: 10.1128/MCB.25.16.7181-7192.2005

FIG. 1.

FIG. 1.

Ligation of SIRPα induces macrophage NO production. (A) Rat NR8383 macrophages were cultured for 18 h. in the presence of control IgG1 (MAb BF5, 20 μg/ml), anti-SIRPα MAb ED9 (20 μg/ml), or LPS (100 ng/ml), and amounts of secreted NO, TNF-α, IL-1β, or IL-6 were determined. (B) NO production in NR8383 macrophages was measured after addition of different intact MAb or corresponding F(ab)2 fragments (all at 20 μg/ml; 18 h of incubation). (C) Effects of control IgG1 (20 μg/ml, ○), anti-SIRPα MAb ED9 (20 μg/ml, •), IFN-γ (20 U/ml, ▵), or ED9 plus IFN-γ (▴) either alone, or in combination with various LPS concentrations, on NO production in rat peritoneal macrophages. (D) TNF-α secretion by peritoneal macrophages (PMph) or NR8383 cells cultured in the presence or absence of ED9 (20 μg/ml) and/or LPS (100 ng/ml) for 18 h (P values were obtained by Student's t test). (E) NO production in NR8383 macrophages triggered by murine CD47-Fc protein (25 μg/ml; 18 h of incubation) or anti-SIRPα MAb ED9 (10 μg/ml). (F) Anti-SIRPα MAb ED9 (10 μg/ml; 18 h of incubation) induces iNOS protein expression in NR8383 macrophages as shown by Western blotting. Experiments were performed at least in triplicate, and results are shown as means ± SD.