FIG. 1.

Integrin- and TGFβ1-mediated spreading of gastric carcinoma cells on fibronectin. (A) TGFβ1 and integrin signaling are required for spreading of gastric SNU16mAd carcinoma cells on fibronectin. The cells were trypsinized, collected, washed with RPMI 1640 containing 1% BSA, kept in suspension for 1 h at 37°C with rolling over, replated on Fn (10 μg/ml)- or PL (10 μg/ml)-precoated dishes, and incubated without or with 5 ng/ml TGFβ1 treatment for 20 h. Phase-contrast images were taken after the incubation. (B) Integrin- and TGFβ1-mediated cell spreading was blocked by Smad7 overexpression. Cells were infected with adenovirus encoding for β-galactosidase (LacZ) or Smad7, an inhibitory Smad. Twenty-four hours later, infected cells were manipulated, as explained above.