FIG. 2.

PKC pathway correlated with the integrin- and TGFβ1-mediated cell spreading. (A) Integrin- and TGFβ1-mediated cell spreading was abolished by PKC inhibition using GF-109203X (GF) and rottlerin (Rot). The cells were pretreated with GF-109203X (12.5 μM) or rottlerin (10 μM) 30 min prior to replating on Fn and TGFβ1 treatment for 20 h. (B) Phosphorylation of PKCδ serine 643 was increased by TGFβ1 treatment for 20 h and correlated with the integrin- and TGFβ1-mediated cell spreading. The cells were manipulated as in panel A. GF-109203X was treated as explained above. After the incubation for 20 h, cell lysates were prepared as described in Materials and Methods. Data shown are representative of several independent experiments.