FIG. 3.
Regulation of expression of cell cycle regulatory proteins by hPNPaseOLD-35 and its deletion mutants. A. HO-1 cells were infected with the indicated Ad at an MOI of 50 PFU/cell for 3 days, and the expression of the indicated proteins in the cell lysates was analyzed by Western blot analysis. B. HO-1 cells were treated as in panel A, and CDK2 activity was assayed using histone H1 as substrate as described in Materials and Methods. The expression level of CDK2 in 5% of the input used for the CDK2 activity assay was determined by Western blot analysis. C. HO-1 cells were infected with Ad.hPNPaseold-35 at an MOI of 50 PFU/cell for 2 days, and immunofluorescence studies were performed to analyze the expression of hPNPaseOLD-35 and p27KIP-1 as described in Materials and Methods.