FIG. 5.
The RPH domain of hPNPaseOLD-35 is required for in vitro degradation of c-myc mRNA. A. Representation of the in vitro-translated products from the indicated plasmids documenting their authenticity. B. In vitro degradation assays were performed as described in Materials and Methods. The expression of c-myc, GADD34, and GAPDH mRNAs was detected by Northern blot analysis. C. Quantification of c-myc/GAPDH mRNA levels in the different groups analyzed in panel B. The data represent the means ± standard deviations of three independent experiments.