FIG. 2.
DSBs and homologous recombination induced by camptothecin depend on replication. (A) Pulsed-field gel electrophoresis showing DNA fragmentation following a 6-hour treatment with camptothecin (200 nM) and/or aphidicolin (3 μM). (B) Homologous recombination measured as the frequency of reversion to HAsT-resistant colonies in S8SN.11 cells following a 6-hour treatment with camptothecin (200 nM) and/or aphidicolin (3 μM). The average (column) and standard deviation (error bars) of three experiments are depicted.