FIG. 1.
Nucleophosmin interacts with the core histones preferentially. (A) Schematic representation of the C-terminal deletion mutants of nucleophosmin with the key to domain organization. (B and C) Interaction of the His6-tagged, full-length (FL) nucleophosmin and its deletion mutants (1 μg) with HeLa core histones (5 μg) (B) or individual recombinant histones (C) was investigated using a Ni-NTA pulldown assay. The beads were loaded on an SDS-12% PAGE gel and visualized by CBB staining. The same was blotted on a polyvinylidene difluoride membrane and probed with antibodies against different histones as indicated.