FIG. 5.

A possible interchromosomal translocation generated upon L1 retrotransposition. Green and black lines indicate the structures of the preintegration sites on chromosomes 4 and 6, respectively. The insertion initiated at an integration site that map to chromosome 6 by endonuclease-dependent TPRT. The undulating and straight black lines represent L1 mRNA and minus-strand L1 cDNA, respectively. The resultant insertion is shown at the bottom of the figure. The filled black rectangle indicates the newly integrated L1. The red line indicates a 336- to 340-bp segment of DNA derived from chromosome 3. Small arrows indicate the positions of PCR primers used to characterize the insertion. PCR demonstrated that the respective junction sequences were not present in naïve HeLa cells and that the insertion is flanked by at least 6 kb of genomic DNA that maps to chromosomes 4 and 6, respectively.