FIG. 3.

Expression of C/EBPβ, C/EBPα, or PPARγ restores adipogenic conversion in KSR1^−/− MEFs. (A and B) Oil Red O staining of KSR1^−/− MEFs expressing (A) C/EBPα and/or PPARγ or (B) C/EBPβ (wild type, LAP, or LIP) untreated or following 8 days of treatment with differentiation medium to induce adipogenesis as described in Materials and Methods. (C) Analysis of MCE in KSR1^−/− MEFs expressing C/EBPβ or vector control by counting cell numbers 0 and 3 days after treatment with differentiation medium. Values are the means of four independent experiments. (D) Western blot analysis of whole-cell extracts prepared at different times during differentiation of KSR1^−/− MEFs expressing C/EBPβ. Lysates were probed with the indicated antibodies to detect induction of each protein during adipogenesis.