FIG. 1.

(A) Western blot analysis of cell lysates from HCT116 and the MnSOD-overexpressing cell lines HCTMnS9 and HCTMnS17. Membranes were probed with an antibody against MnSOD and with an anti-CuZnSOD antibody (loading control) as indicated. (B) The extent of MnSOD overexpression in the HCTMnS9 and HCTMnS17 cell lines was measured by densitometric analysis of three different Western blots. In the diagram the overexpression value refers to the parental cell line HCT116. (C) Growth curves of HCT116 and the HCTMnS9 and HCTMnS17 cell lines. Cell numbers are averages from three independent experiments ± standard errors. (D) Amounts of apoptotic cells in HCT116 and HCTMnS9 cells were measured according to a method of Nicoletti et al. (28) in three independent experiments. Results are depicted as means ± standard errors.