FIG. 1.

Dbf4 mutants. Full-length Dbf4 (Dbf4FL) and mutants used in this study are depicted. The N and M motifs, which are highly conserved among eukaryotes, are illustrated in gray. Numbers below indicate corresponding amino acid position, and the two regions that bind Cdc7 (19) are indicated by solid bars. The single amino acid change from proline to leucine at position 277 resulting from the dna52-1 point mutation is indicated by an asterisk (Dbf4ts-FL). DNA cassettes encoding these Dbf4 variants were used for the construction of both pEG-202-based (1) and pCM190-based (15) expression vectors.