FIG. 5.

Dbf4ts-FL confers resistance to both MMS and HU. DY-1 and DY-2 cells, transformed with pCM190-Dbf4-FL, pCM190-Dbf4ts-FL, or empty pCM190 (V), were spotted in 10-fold dilution series onto selective media plates containing the indicated concentrations of MMS and HU, as described in Materials and Methods. Each plate series was incubated at 23°C or 30°C for 3 days, as indicated. It should be noted that reduced DY-1 cell growth at 23°C relative to 30°C likely reflects a slower cell cycle (see 0 mM HU and 0% MMS panels) rather than increased sensitivity to genotoxic agents at the lower temperature.